Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands
Cornelissen, Marion. Zorgdrager, Fokla. Blom, Petra. Jurriaans,
Suzanne. Repping, Sjoerd. van Leeuwen, Elisabeth. Bakker, Margreet.
Berkhout, Ben. van der Kuyl, Antoinette C.
Laboratory of Experimental Virology, Department of Medical Microbiology,
Academic Medical Center, University of Amsterdam, Amsterdam, The
Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The
PLoS ONE [Electronic Resource]. 5(8):e12040, 2010.
Source: NLM. PMC2919391
BACKGROUND: Xenotropic murine leukaemia virus-related virus (XMRV) is a
recently discovered human gammaretrovirus with yet unknown prevalence and
transmission route(s). Its presence in prostate stromal fibroblasts and
prostatic secretions suggests that XMRV might be sexually transmitted. We
chose to study a compartment closely connected to the prostate, a location
where XMRV was detected in independent studies. Seminal plasma samples
from HIV-1 infected men were examined as they have an increased
probability of acquiring sexually transmitted pathogens.
METHODOLOGY/PRINCIPAL FINDINGS: We studied the prevalence of XMRV in 93
seminal plasma samples of 54 HIV-1 infected men living in The Netherlands
with a nested PCR amplification specifically targeting the XMRV gag gene.
As a control for the presence and integrity of retrovirus particles, HIV-1
was amplified from the same samples with a PCR amplification targeting the
env gene of the virus, or HIV-1 was quantified with a real-time PCR
amplifying part of the pol gene. CONCLUSIONS/SIGNIFICANCE: Although HIV-1
was amplified from 25% of the seminal plasma samples, no XMRV was
detected, suggesting that either the prevalence of XMRV is very low in The
Netherlands, or that XMRV is not naturally present in the seminal plasma.