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Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The Netherlands

Nov
2010
10

posted by kkaneshi | |

Authors 

Cornelissen, Marion.  Zorgdrager, Fokla.  Blom, Petra.  Jurriaans,
  Suzanne.  Repping, Sjoerd.  van Leeuwen, Elisabeth.  Bakker, Margreet.
  Berkhout, Ben.  van der Kuyl, Antoinette C.
Institution
  Laboratory of Experimental Virology, Department of Medical Microbiology,
  Academic Medical Center, University of Amsterdam, Amsterdam, The
  Netherlands.
Title
  Lack of detection of XMRV in seminal plasma from HIV-1 infected men in The
  Netherlands.
Source
  PLoS ONE [Electronic Resource].  5(8):e12040, 2010.
Other ID
  Source: NLM. PMC2919391
Abstract
  BACKGROUND: Xenotropic murine leukaemia virus-related virus (XMRV) is a
  recently discovered human gammaretrovirus with yet unknown prevalence and
  transmission route(s). Its presence in prostate stromal fibroblasts and
  prostatic secretions suggests that XMRV might be sexually transmitted. We
  chose to study a compartment closely connected to the prostate, a location
  where XMRV was detected in independent studies. Seminal plasma samples
  from HIV-1 infected men were examined as they have an increased
  probability of acquiring sexually transmitted pathogens.
  METHODOLOGY/PRINCIPAL FINDINGS: We studied the prevalence of XMRV in 93
  seminal plasma samples of 54 HIV-1 infected men living in The Netherlands
  with a nested PCR amplification specifically targeting the XMRV gag gene.
  As a control for the presence and integrity of retrovirus particles, HIV-1
  was amplified from the same samples with a PCR amplification targeting the
  env gene of the virus, or HIV-1 was quantified with a real-time PCR
  amplifying part of the pol gene. CONCLUSIONS/SIGNIFICANCE: Although HIV-1
  was amplified from 25% of the seminal plasma samples, no XMRV was
  detected, suggesting that either the prevalence of XMRV is very low in The
  Netherlands, or that XMRV is not naturally present in the seminal plasma.